Teenage Drinking Essay Example for Free

Teenage Drinking Essay For the past decade, there has been a major problem with underage drinking in this world. Many of teenagers have been able to buy consumed drinking without the appearance of their parents. Today, many teenagers experience different things in the world. Whether it is their first date or first day in high school, teens are always eager to try something different or new. One of the things that teenagers try is drinking alcohol. Unfortunately, underage drinkers often abuse alcohol. Many reasons why underage teens are consuming alcohol because there were alcoholics in the family, peer pressure, stress and family problems. When growing up with an alcoholic, the kids mind thinks that it is normal to drink non-stop. The emotional and psychological scars that children can develop in alcoholic homes can be so deep that they can last well into adulthood. If you were raised in a home with alcoholism or addiction, you may find that some of the following characteristics are very familiar(Dr. Jan Woititz). People tend to become isolated, fear people and authority figures become approval seekers, be frightened of angry people, be terrified of personal criticism, become alcoholics, marry them or both view life as a victim have an overwhelming sense of responsibility, be concerned more with others than themselves, feel guilty when they stand up for themselves, become addicted to excitement, confuse love and pity love people who need rescuing, stuff their feelings lose the ability to feel, have low self-esteem, and judge themselves harshly. Those are just few of the effects of alcoholic problems in the family. Studies state that people look to alcohol because of economic, job stress or marital discord. In humans between certain types of alcoholism and adverse early childhood experiences. High levels of stress may influence drinking frequency and quantity. This relationship between stress and drinking even is stronger when alternative coping mechanisms and social supports are  lacking. Finally, when individuals believe that alcohol will help to reduce the stress in their lives, alcohol is most likely to be used in response to stress. Drinking appears to follow stress but some evidence also links excessive drinking to the anticipation of a major stress or even during times of stress (BRESSERT). Stress can lead to being a alcoholic because the person is so tired of being in a state of stress so they look for something that can calm them down so they turn to either weed or alcohol. They drink as much as they could until it goes away but in reality the stress does not go away. So if it do not work then that means they going to keep looking towards liquor to get rid of it. This drinking can make their way of thinking change. Over time, heavy drinking interferes with the neurotransmitters in the brain that are needed for good mental health. So while alcohol may help deal with stress in the short term, in the long run it can contribute to feeling of depression and anxiety and make stress harder to deal with (Eva Cyhlarov). Joseph A. Califano, Jr says The media focuses on illegal drugs [ such as ] heroin, but thats the tail. The dog is alcohol and the dog is really biting our kids (69). This can cause you to hurt others, get you into illegal trouble, and damage your relationships. Alcohol can damage the young brain, interferes with mental and social development, and interrupts academic progress. Alcohol is attracts teenagers easily and leads to the three leading causes of teen death: accidents, homicide and suicide. You can also harm your body now and when you grow up and also get you addicted. When drunk people tend to not notice anything so they start fights, unprotected sex, driving drunk and heavy hangovers. People have died from drinking on the beach and like to go swimming which leads to death. It is said from Anthony that people dont die from smoking weed, they die from drinking alcohol(70). It seems to be true because you cannot die from a plant but you can die from something that is mix with ma ny chemicals. They can die from intense alcohol intoxication. Why do teens drink? Teens seem to look towards drinking as a pleasure but what they do not notice is messing up their body. Even though they know its bad for them, they insist that alcohol isnt really a drug, however kids  know that beer, wine, and liquor can you into big trouble. It is said by CASA that 8th graders used 47.0% of alcohol, 10th graders used 66.9% and 12th Graders used 78.4%. Used in past year, 8th graders are 38.7%, 10th graders are 60.0%, and 12th graders are 71.5%. Used in the past months, 8ths graders are 19.6%, 10th graders are 60.0% and 12th graders are 48.6%. Seventy-two percent of college students report that they used alcohol at least once within the 30 days prior to completing the Core survey. Within the last year, 84 percent of students report they drank alcohol (Alcohol and College Students Drinking Fact Sheet, 1). 82% of students under age 21, using alcohol within the past year. Student athletes are more often the heaviest drinkers in the overall student population. Half of college athletes (57 percent of men and 48 percent of women) are binge drinkers and experience a greater number of alcohol related harms than other students. College athletes are also more likely than other students to say that getting drunk is an important reason for drinking(Dying to Drink by Henry Wechsler, Ph.D.). Seventy-eight percent of college athletes report that they used alcohol on at least one occasion in the past 30 days prior to completing the Core survey. Within the past year, 88 percent of student athletes report using alcohol. One in five athletes believe others students drinking adversely affects their involvement on an athletic team or in other organized groups. Some athletes do not think of the repercussions while they are out with fellow teammates, drinking a limitless amount of alcohol. They are caught up in the moment and want to be cool with friends and associates. They don’t think how this is going to affect their upcoming game or practice. Some athletes believe they can handle excessive drinking and performing well in games. There thought pattern is party hard right now and worry about practice or the game later. This is a poor thought pattern that will always harm their performance. Athletes know alcohol affects their system in a negative way, yet they still consume large amounts(Nelson and Wechsler1 2001). Colleges and pro-football leagues look at this kind of things. They have a wide-range programs that can track and also test 100% if someone is under the influence of alcohol and drugs. Today, as you may have read many teenagers are under the influence of alcohol in the stages of high school and college. There were many studies  that were conduction that gave us information about the average student alcoholics or up and coming alcoholics. There were many effects of alcoholic that people did not understand. These kids do not understand what it can do to your life and physical-self in the future. They wont understand the outcomes until it hits them clearly in the head.

Assertions and Audit evidence Essay Example for Free

Assertions and Audit evidence Essay 1. The allowance for doubtful accounts is fairly presented in amount. * Valuation or allocation assertion 2. All accounts payable owed as of the balance sheet date are included in the financial statements. * Existence or occurrence assertion 3. All purchase returns recorded in the general ledger are valid. * Existence or occurrence assertion 4. The allowance for doubtful accounts is fairly presented in amount. * Valuation or allocation assertion 5. All accounts payable owed as of the balance sheet date are included in the financial statements. * Completeness assertion 6. All purchase returns recorded in the general ledger are valid. * Existence or occurrence assertion 7. A retail client values its inventory using the retail method of accounting. * Valuation or allocation assertion 8. A construction client uses the percentage of completion method for recognizing revenues. * Existence or occurrence assertion 9. A client has a defined benefit pension plan and does not have competent employees to write footnote disclosures. * Presentation and disclosure assertion – classifications and understandability 10. Slow-moving items included in inventory have been properly identified and priced. * Valuation or allocation assertion 11. A client financed the acquisition of assets using preferred stock that pays a 3 percent dividend and must be redeemed from the shareholders next year. * Rights and obligations assertion 1. Bank statement sent directly to the auditor by the bank. (1) – Most reliable 2. Creditor monthly statement obtained from clients files. (2) – Reliable, but subject to possible forgery 3. Vouchers in clients unpaid voucher file. (4) Least reliable 4. Duplicate sales invoices in filled order file. (4) Least reliable 5. Time tickets filed in payroll department. (4) Least reliable 6. Credit memo in customers file. (4) Least reliable 7. Material requisitions filed in storeroom. (4) Least reliable 8. Bank statement in clients files. (2) Generally reliable 9. Management working papers in making accounting estimates. (4) Least reliable 10. Paid checks returned with bank statement in (1) above. (3) Somewhat reliable 11. Letter in customer file from collection agency on collectability of balance. (2) Generally reliable 12. Memo in customer file from treasurer authorizing the write-off of the account. (4) Least reliable

Marketing Plan for an Updated Product

Marketing Plan for an Updated Product A marketing plan is typically designed to establish a framework for management to use as they pursue the marketing and sales objectives. It should be built on the results of the market research and the specific value proposition of a product or service. In general, a marketing plan is a shorter form of a business plan that has a limited scope and marketing emphasis. I have chosen UK chocolate confectionary market to present a market plan for an updated product. Market Overview: Chocolate sales in Europe have been growing steadily. The UK confectionery market achieved year-on-year growth for the last five to six years to reach a value of  £4.53bn i.e. the sales have increased by 13%. The overall European chocolate sales increased by 15% and the United Kingdom itself accounts for nearly 30% of European chocolate consumption. Product Overview: So far the need for innovation and the product development techniques has been explained. On the basis of these discussions I would like to use the opportunity to develop a new product in the confectionary market. Taking the existing products of Cadbury such as Cadbury Picnic, Cadbury Crunchie and Boost chocolate bars as well as Cadbury Crà ¨me Eggs, I have thought of updating Cadbury chocolate bars by producing a new chocolate bar and the Crà ¨me Eggs by producing another egg shaped product. The analysis of innovation need suggests that launch of new product can help Cadbury to attract more customers and thus increase market share in a highly competitive market of chocolate confectionary. Assuming the results obtained from the techniques for new product development and analyzing the market overview, I would say that a new product can be launched in the market and thats why I have thought of updating Cadburys existing products into other new products. This Cadburys new chocolate bar will be known as CADBURY LEMON BAR and the new egg shaped product will be known as CADBURY CHOCOLATE LEMONS. The bar name has been selected because this chocolate bar will be having an internal layer of sweet lemon tart and externally it will be covered with a mix of chocolate and fine grains of peanuts. N.B: (In the report the word Cadbury Lemon means both the updated products i.e. Cadbury Lemon Bar and Cadbury Chocolate Lemons). Market Segmentation: Understanding the needs and desires of consumers creates the opportunity for new products to be developed and brought to the market, meeting these needs and generating incremental sales. Chocolate confectionary is a mass market attracting customers and consumers of almost every age group and gender, but those who like eating chocolate. The target market for both the Cadbury Lemon products can include children of age 5 to 13 years in terms of consumers and the parents to pay for the product in terms of customers. Further more it can be expanded from children of age 14 to young people and then onwards to aged people (65+) as long as they keep their interest in their chocolate eating habit. The product would also be suitable for vegetarians and further more it would be excluded of any religious issues such as alcohol free etc. In terms of income and price of the product there isnt any need to be selective to choose a particular group of audience as everybody can easily afford to buy a b ar of chocolate or a pack of chocolate sweets i.e. Cadbury Lemon Bar and Cadbury Chocolate Lemons. Snacking Today: Snacking today is the second largest segment in the UK confectionary market with a share of over 28%. Cadbury Lemon chocolate bar is such a product which is mainly bought for immediate personal consumption. The target market of this product would be people who will buy a chocolate bar and eat it. It does not identify any particular segment but includes everybody having an access to the stores and retail shops where they will find the product, buy it and consume it. For Home: The products in this category are those which are bought for consumption sometime later. Thus in this category both the products i.e. Cadbury Lemon Bar and Cadbury Chocolate Lemons will fit properly in this category of products. These two products would target people who like filling their cupboards with chocolate to consume them for different purposes e.g. filling lunch boxes or as a treat after evening meals. For home is a category which is more about the products to be eaten by the customers i.e. both male and female partners, their children and parents and as well as by other consumers such as visitors or guests visiting at different times e.g. evening tea time and so on. Sharing Occasions: This segment represents an emerging trend towards the sharing of confectionary at formal occasions. This is an emerging segment for products which are traditionally packed in larger pack formats such as tins, large boxes and cartons. They are purchased not as gifts but also for informal sharing on specific occasions e.g. the Cadbury Chocolate Lemons can be purchased as a gift for someones birthday and Cadbury Lemon Bar can act as a sharing product at a party or just at small gathering of friends. This is how another segment of the market can be differentiated. Seasonal Giving: Seasonal giving is the third largest segment of the overall confectionary market. The rate of growth of this segment has led to an increase of 3.8% in sales and an increased share of the overall confectionary market as 17.6%.This segment includes products bought to give to someone else as well as a larger number of seasonal lines and Cadbury Chocolate Lemons in different packs would best fit in this category. Seasonal confectionary is mainly sold during the run up to Christmas and Easter and is a traditional gift at these times of the year. Along with this other seasonal occasions can include Halloween, Mothers Day and Valentines Day. Competition Overview: The UK confectionary market is facing a very strong competition among the companies in this market. Thus Cadbury Lemon will also have to tackle such circumstances of competition from different major competitors including Nestle, Kit Kat, Mars, Maltesers and others. Moreover, rapid product growth at the mass end and the premium end of the European chocolate market is adding competitive pressures. Smaller companies are making chocolate products for niche markets, such as chocolates for people who want to avoid dairy products, chocolates for people who are diabetic and chocolates for people who prefer natural or organic flavourings and ingredients. Further, established companies are constantly introducing variations and other favourites to satisfy customers variety-seeking behaviour and encourage loyalty. Although Cadbury owns a good market share but still there is a need to be aware of the competitors and the situations which may cause hindrances for the new product. SWOT Analysis: Cadbury has been in the confectionary business for a long time and holds a strong market share. Thus the company has been using its strengths to always go for producing new products as well as updating the existing ones. National advertising and sales promotion support sales of Cadbury and for instance, Cadbury has been spending heavily to launch new chocolate products or building sales during Easter or other holiday periods. There might also be some weaknesses that could be associated with it. Most of the people see chocolates as an affordable luxury and therefore buy such products for themselves and also for gifts. People are adopting the habits of giving chocolate as gifts on different occasions like Christmas, Valentines Day and Birthdays etc which has increased the opportunities in terms of increase in demand for the product. However the threats are always there when a company launches a new product. In addition to the major luxury chocolate makers with established brands, natio nal advertising campaigns and sizable market share, many smaller and local chocolate makers are attracting loyal customers. But on the whole, so far, the UK confectionary market for Cadbury seems to be having the opportunities to grow and increase the market share. Goals and Objectives: For this marketing plan I would say that the goals and objectives are simply the facts describing where the company i.e. Cadbury wants to be. The goals and objectives can be divided into two parts. Sales Goals: For many organizations the ultimate goal of the marketing plan is the effect it will have on the bottom line. Sales goals would be in terms of the sales figures estimated to be achieved. Until 2005, Cadbury has been earning great sums of profits and the further need is to increase the profits or maintain the profitability of the company up to a level at least meeting the required levels. Marketing and other Objectives: The marketing objectives for Cadbury Lemon should address each group in the target market in terms of meeting customer satisfaction and their interests in buying the product. The market objectives for Cadbury Lemon should fulfill the same elements as set for other brands. It includes continuing high Corporate and Social Responsibility standards through actions and brands, delivering superior shareowner performance, ensuring the capabilities are best in the market, focusing on creating a cohesive and talented workforce through encouraging inclusiveness and increasing the diversity of our people. Hence the product would only be said to be successful if these goals and objectives would be clearly identified and followed properly. Pricing Strategies for Cadbury Lemon: Pricing in general meanings means how much to charge for a product or service? Pricing is one of the four Ps of the marketing mix. The other three aspects are Product Management, Place (distribution) and Promotion. Penetration Pricing: Penetration pricing is the pricing technique of setting a relatively low initial entry price, a price that is often lower than the eventual market price. The expectation is that the initial low price will secure market acceptance by breaking down existing brand loyalties. Penetration pricing is most commonly associated with a marketing objective of increasing market share or sales volume, rather than short term profit maximisation. Competitive Pricing: The competitive pricing strategy is used by around a quarter (27%) of organisations. It is the most usual form of pricing which is based on evidence from the market where product prices are determined by reference to the prices of competitive products. A sound appreciation of competitive actions, especially prices, is necessary for the most effective strategies to be formulated. The most effective marketing manager will, however, try to develop an understanding of the various competitive positions based on an appreciation of the customer needs. Distribution: Like Pricing, distribution is another important part of marketing mix which needs businesses to carry out detailed analyses in order to distribute their products/services easily and efficiently to their customers. A distribution business is the middleman between the manufacturer and customer.. As far as distribution of confectionary products is concerned, the best and appropriate options would be the following. (Method 1) (Method 2) Producer Producers Retailers Whole Sellers Customers Retailers Customers Communication: For Cadburys updated products i.e. Cadbury Lemon Bar and Cadbury Chocolate Lemons I would like to recommend following communication techniques for the product promotion. TV Advertising: Importantly I would like to recommend using TV as the most appropriate tool of communication. Cadbury has already been sponsoring Coronation Street for a long time. So when people will be watching the programme then in every break the advert will be presented and people will see how the new product has been developed. This technique will pass the message to the right target market and thus will create awareness among public. Bill Boards: Bill boards are though to be another important tool of sending the message across the target market and overall to every one. Bill boards will be displayed at certain points which will clearly explain what the product looks like and how has it been developed. People while traveling will get attracted towards the product being displayed on bill boards and thus will get the message. Hence hopefully this technique is thought to be equally appropriate to create awareness among public and then leading them to further stages of decision making and purchase of product. Transport: Compared to bill boards, transport will be taken as a source of moving message across people. Thus this is another technique which holds an equal importance as TV advertising and bill boards. Transport including buses, trams, trains and to some extent taxis are thought to be quite suitable for making people aware of the product. The product will be displayed with its features and it differences from other products on the above mentioned transports. People when traveling on these transports or following them, will get attracted towards the printed message. So this is how it will help Cadbury to increase their profits when the sales will go up. Sales Promotions: One of the below the line techniques that I think would help to increase sales is sales promotion. It can be done in a way that Cadbury can encourage customers to buy Cadbury Lemon by offering the products at trial prices or another way could be that first few millions of products would be given free without any charges. Similarly when the products would be sold through a third party e.g. retailers then it can also be looked t providing point of sale material, or special promotions aimed at encourage the distributors to sell or stock more products, so that it can help to increase the amount people will use our product. Monitoring and Reviewing Marketing Performance: The organisations who manage to be both efficient in their operations and effective that they deliver what their customers want, they enjoy the benefits of their business in real sense. On the other hand those who are neither efficient nor effective lose their market share and at last come out of the business. Financial Measures: Profit: Most of traditional financial measures concentrate on profit and it goes without saying that profit is essential to the long-term survival of any business no matter what size or shape. The performance of Cadbury Lemon could easily be judged by analysing the difference in the profits of the company. If the companys profits have gone up then it can be assumed that the product has been successful in the market and that it has helped to meet the companys objectives in terms of achieving the target profit. Meeting the financial targets is very important for every business. Companies invest a lot of money in carrying out Research and Development to make their products effective and efficient in the market. So a successful launch of Cadbury Lemon and the analysis of profit would clearly help Cadbury to make decisions about whether the product should be kept in market and let it become a cash cow or pull it out before it becomes dog. Return on Sales: Another area that gives good and bad signals to the businesses about their performance is sales. Return on sales can tell how well a product or service is performing in the market. This is how Cadbury Lemons sales will help to see what the demand for the product is. This can be analysed, whether the consumers have liked the idea of developed product and are doing repeat purchases or the product have failed to satisfy customers needs. Thus higher the sale levels will be greater would be the customers satisfaction, which will result an increase in Cadburys market share. Now lets have a look how non-financial measures can take part in reviewing Cadbury Lemons performance. Barriers to Marketing Planning: In order to overcome any problem the first and important requirement is appropriate monitoring of the problem and see how easily and effectively can a business overcome a barrier. As long as the proper control measures are installed there will be no more problems that can interfere. Taking a look on previously described external barriers; organisations are needed to take complete analyses of the factors that could influence their strategic implementation process. The political as well as legal factors especially Government policies related to taxation, employment, health and safety and other issues can be influential for the organisations. So for organisations, it is necessary to keep in mind the Governments rules and regulations in order to take step in any part of business strategy. Accordingly it would be important for managers to detect changes early so as to respond effectively. Similarly an early forecast of economic factors is also important so that recommendations related to future can be made. Technological barriers to some extent could be considered as slow acting barriers because technology does not change everyday. However for Research and Development purposes technology plays a vital role in giving the information to businesses that, then, is to be used for developments processes. The internal marketing, being an integral part of successful strategic implementation, involves all the processes necessary to carry the message of the strategic plan inside to the various audiences that compromise the organisation. The internal marketing is equally important as that of external marketing and thus it needs to be analysed fully in order to eliminate errors. Like external marketing, internal marketing also requires a good and appropriate understanding of the needs and motivations of the target audiences to be successful. The organisations should be designed in such a way that the communication between top and the bottom layers is quick and detailed. Keeping everyone informed about any changes going on within an organisation can help to take ideas and views from internal bodies which could then further be utilised in the process of final decision making. In order to analyse that how well a marketer is performing, the information can be obtained with the help of a marketing feedback. Although the information and feedback on a plans progress is can never be 100% accurate but it does act to both reduce the uncertainty in planning and improves the quality of action. Conclusion: Hence in this report the need for innovation for organisations has been explained. Product development techniques have been discussed in detail with reference to the confectionary market. A marketing plan has been presented which is covering all the aspects related to market review, competition, pricing, distribution and communication. The measures to review performance have also been talked about. And finally the barriers and the suggestions to overcome these barriers have also been explained in detail.

How to Organize and Run Effective Meetings Essay -- Workplace Meetings

INTRODUCTION Workplace meetings can be considered as essential but one of the most inefficient components of a corporate environment. Project managers can significantly influence the project performance by proactively influencing and controlling the dynamics of project meetings. This can ultimately generate highly valuable outputs in achieving team integration and consensus. ISSUES WITH MEETINGS Experts widely concur that there are too many meetings than required and most meetings are a waste of valuable time (American Marketing Association, 2011). Meetings can tend to get long and futile. Unfortunately, most of the meetings are hampered by vague agendas and directionless discussions. An attitude of low commitment creeps in and leads to dwindling participation. POSSIBLE SOLUTIONS Detrimental effects of unproductive meetings on a project are generally overlooked. In this essay I will attempt to examine meeting dynamics, including the five Ps (purpose, product, participants, process, and probable issues) and develop a reference cookbook for organizing and running meetings. INITIATION PHASE Strategy Meeting: The purpose is to analyze if a suggested business need fits with the organization’s strategic framework and vision (Tammy Adams, 2008). Products of this meeting include developing an initial draft of the business case, aligning it to overall organizations strategies. The participants should be low in number and include senior executives, sponsors and business process experts who would analyze the direction of the project. The process should focus on dividing the meeting in short segments and focus on individual items at a time. The possible issues could stem from to not presenting the complete data for the busine... ...p://www.the-program-manager.com/project-management/how-to-organize-an-effective-meeting/ PMI. (2004). Quickbooks. (2009, December 16). Project Closeout Meeting Agenda - DOC. Retrieved 4 12, 2014, from docstoc: http://www.docstoc.com/docs/19614443/Project-Closeout-Meeting-Agenda Schwalbe, K. (2012). Information Technology Project Management. Boston MA: Cengage Leaning. Tammy Adams, J. M. (2008). Project Meeting Facilitator: Facilitation Skills to Make the Most of Project Meetings. San Francisco: Jossey-Bass. UGM Consulting. (2009, July 23). Strategy Design and Facilitation: The UGM Consulting Approach. Retrieved 4 12, 2014, from UMGConsulting: http://www.ugmconsulting.com/Effective%20strategy%20design.pdf Vick, J. (2012, January 12). Project Management: How to Run Team Meetings. Retrieved 4 12, 2014, from Youtube: https://www.youtube.com/watch?v=xW0CjH95K3Q

Protein Thermal Stability Essay -- Scientific Research, Amino Acids

Proteins are organic polymers made up of chains of amino acids and are crucial material in many biological functions (Reece and others 2011). There are twenty basic amino acids, eight of which are essential to the adult human diet and must be consumed rather than synthesized inside the body. These essential amino acids are isoleucine, leucine, tryptophan, lysine, valine, phenylalanine, methionine, and threonine, with an added requirement of histidine in the diet of children (Potter and Hotchkiss 1995). Nonessential amino acids can be synthesized from these, and all amino acids are used as monomers to construct proteins which perform numerous important functions in the body (Reece and others 2011). Most amino acids exist as isomers and are given a designation based on whether they rotate a plane of polarized light to the right or to the left, known as dextrorotatory â€Å"D† or levorotatory â€Å"L† isomers, respectively (Al-Holy and Rasco 2007). It should be noted that only L amino acids are used in protein synthesis (Weber and Miller 1981). The biological functions of proteins include increasing the rate of biochemical reactions in the form of enzymes, moving important substances to where they are needed in the form of transport proteins, regulating the body in the form of hormones, allowing the body to move in the form of contractile proteins, and providing support in the form of structural proteins (Reece and others 2011). In addition, proteins have a great deal of functionality in foods, including providing essential amino acids as well as energy, viscosity, texture, water holding capacity, foaming and emulsification properties, and allowing gel formation (Culbertson 2007). As mentioned above, essential amino acids provided by th... ... p. Reece JB, Urry LA, Cain ML, Wasserman SA, Minorsky PV, Jackson RB. 2011. Campbell biology. 9th ed. San Francisco: Cummings-Pearson. 1263 p. Siegwein AM, Vodovotz Y, Fisher EL. 2011. Concentration of soy protein isolate affects starch-based confections’ texture, sensory, and storage properties. J Food Sci 76:E422-8. Sorgentini DA, Wagner JR, Anon MC. 1995. Effects of thermal treatment of soy protein isolate on the characteristics and structure-function relationship of soluble and insoluble fractions. J Agric Food Chem 43:2471-9. Thompson LD, Dinh T. 2009. Food proteins-protein isolation and thermal stability. FDSC 4303/5303 food chemistry laboratory manual. Lubbock, Tx.: Texas Tech University, Department of Animal and Food Sciences. Weber AL, Miller SL. 1981. Reasons for the occurrence of the twenty coded protein amino acids. J Molecular Evolution 17:273-84.

Essay --

Expression System: Combination of host and expression vector and its DNA clone, induce the function of foreign gene within host cell that yield proteins in larger amount in called as expression system. pET Expression System: pET is a vector that is used for cloning of desired proteins within host cell. Introduction: pET expression system is widely used method for cloning for creation of No. of clones for most wanted proteins. For this purpose expression vector or recombinant vector is introduced within host cell. This recombinant vector carry all gene essential for manufacturing of proteins, having promoter suitable for host cell, it also carry terminal sequence for transcription, and also have ribosome binding sites. One pET expression system was introduced by W.F. Studies and B.A. Moffatt in 1986 that was expression system of T7 polymerase. Two methods were used for stability of T4 RNA polymerase. One of the method uses lambda bacteriophage for induction of coding gene for T4 RNA polymerase and another method involved in direct insertion of gene within chromosome. It is most used method due to production of proteins in large quantity, that present in T7 promoter and have only binding sites for RNA polymerase. (Moffatt. B.A, et. al. 1986) Control of levels of Basal Expression: There are six vector-host unions that are produced by pET expression system. These are essential because only one condition is unfavorable for protein that are targeted. (Resenberg. A.H, et. al. 1987) Host Strains: In non-expression host, after production of plasmids, they are interconverted into T7 polymerase gene (ÃŽ »DE3 lysogen) that carry host to express target protein. The lac UV5 promoter control the gene of T7 RNA polymerase in ... ...ide bond in cytoplasm is induced by strains of OrigamiTM. Rapid: †¢ System that uses E.coli gives results rapidly. †¢ Other vectors provide appropriate restriction sites that are used for subcloning. †¢ Targeted proteins are purified in single step, methods that contain no antibody are selected. Versatile: †¢ Fusion tags of N-Terminal and C-terminal are preferred to purify, purify and locate. †¢ Replication originated at f1 in sequencing and mutagenesis. †¢ Sites for multiple cloning are of wide range. Complete: †¢ Number of useful products †¢ Various shapes of systems. (Imotaka. H. et. al. 2009) Collusion: It is concluded that pET expression system is helpful in synthesizing of proteins. It induce transcription as well as translation. pET vector inserted within host cell carry desired gene to be cloned. pET expression system help in cloning of desirable gene or product.

Research proposal for Risk Management Essay

Introduction: The increasing pace of change, customer demands and market globalization all put risk management high on the agenda for forward thinking companies. Risks cause cost overrun and schedule delay in many projects. The effectiveness of risk management becomes an important issue in project management. To make risk management more efficient and effective, all parties must understand risk responsibilities, risk event conditions, risk preference, and risk management capabilities (Wehrung et al. 1988; Al-Bahar and Crandall 1990) Different parties involved in a construction project face a variety of uncertain factors. These factors can be compiled under the category of risk. Making decisions on the basis of assumptions, expectations, estimates and forecasts of future events involves taking risks. Risk and uncertainty characterize situations where the actual outcome for a particular event or activity is likely to deviate from the estimate or forecast value (Raftery 1994). The definition of risk management varies; risk management is generally defined as, ‘A formal orderly process for systematically identifying, analysing, and responding to risk events throughout the life of a project to obtain the optimum or acceptable degree of risk elimination or control’ (Al-Bahar & Crandall 1990). The objective of my research is to present the perception of typical Chinese contractor towards construction risk. The result of surveys should further clarify the current perception of contractors regarding current construction circumstance in the industry. The data and information will be collected from published statistics, individual interviews and questionnaires, and the data will be used to compare with previous published surveys. The purpose of this comparison is  to identify perception and trends in construction risk management. A basic understanding of current attitudes and trends therefore will be indicated. Then I will adopt some appropriate case to enhance my understanding of my view point. The following proposal presents the ‘What’ and ‘How’ of my study, using an integrated approach to study risk and its management in Chinese construction industry. Brief introduction of Chinese construction industry China’s economy has shown remarkable growth over the past several years and many foreign economists predict a healthy growth in the near future. According to the Chinese Statistical Yearbook 2002, China’s construction industry achieved 646.2 billion yuan in total output value in 2001, accounting for 6.7 percent of the nation’s gross domestic product (GDP). China’s construction industry realized 704.7 billion yuan in total output value in 2002 — an increase of 10.54 percent from the previous year’s 637.5 billion yuan — accounting for 6.9 percent of the national GDP. Construction enterprises reached 45,893 by the end of 2001, employing 21,106,600 people and generating 1,536.156 billion yuan in output value and 402.357 billion yuan in added value. Areas under construction totaled 1,883,286,800 square meters; completed areas covered 976.99 million square meters. Profits totaled 29.439 billion yuan in the year, with taxes at 49.903 billion yuan. Of the enterprises, 44,997 were Chinese with 20,986,800 staff members. The companies reaped 1,518.595 billion yuan in output value in 2001 and 398.022 billion yuan in added value. Areas under construction numbered 1,874,480,800 square meters and completed areas completed covered 972.532,300 square meters. Profits reached 28.83 billion yuan, with taxes at 49.392 billion yuan. (China Statistical Yearbook, 2002) Recent trends of risk management in Chinese construction industry For years, the Chinese construction industry has had a very poor reputation for coping with risk, with many projects failing to meet deadlines and cost targets. This can be traced to many causes as Lu mentioned, including: Technical, quality or performance reasons, such as employment of inexperienced designers, changes to the technology used or to industry standards during the project. Organizational problems, such as cost, time and scope objectives that are internally inconsistent, lack of prioritization of projects, inadequacy or interruption of funding, and resource conflicts with other projects in the organization. External problems, such as shifting legal or regulatory environment, poor geological conditions and weather, force majeure. Project management, such as poor allocation of time and resources, inadequate quality of project plan, and poor use of project management disciplines. (Lu 2003) Risk management is still new to China; it was introduced and applied in construction industry for a relative short period. Survey showed that Chinese contractors are often responsible for most risk factors, meanwhile, the use of risk analysis techniques for managing and controlling risk are generally low among the Chinese construction industry. Basically, most contractors still resort to the subjective judgment and practice experience for perceiving and managing risks. However, according to Chinese Statistical Yearbook 2004, situation was improving. During the Tenth Five-Year Plan, China’s construction industry has so far enjoyed a good opportunity for development, more and more parties involved in the construction projects raised their perception of risk and the attitude towards risk management improved. (China Statistical Yearbook, 2004) Literature Review The literature review in this part is mainly about the identification of risk resources, classification of risk, perception of risk and basic theory of risk management as well. Sources of risk There are many resources of risk that an organization must take into account before a decision is made. It is therefore important that these sources of risk are identified allowing the necessary analysis and response to take place. According to Merna and Smith, sources of risk to business from projects including as follows: political, environmental, planning, market, economic, financial, natural, project, technical, regulatory, human, criminal, safety, legal. (Merna and Smith 1996). A source of risk is any factor that can affect project or business performance, and risk arises when this is both uncertain and significance in its impact on project or business performance. Classification of risk It is helpful to try to categorize risk associated with projects both as a guide to identification and to facilitate the selection of the most appropriate risk management strategy. Merna and smith proposed one method to separate the more general risks which might influence a project but may be outside the control of elements; these are referred to as global and elemental risks. Global risks originate from sources external to the project environment. They are often predictable but not always controllable. Global risks can be subdivided into four sections: political, legal, commercial and environmental risk. Elemental risks are those risks associated with elements of the project, namely implementation risks and operation risks, and for some projects there will be financial risks and revenue risks. These risks are more likely to be controllable or manageable by project parties. (Smith 2003) Perceptions of risk Different people will respond to seemingly similar risky situation in very different ways. Risk perception has a crucial influence on risk taking behavior. The perceived importance attached to decisions influences team  behavior and the consequent implementation methods. Subjectivity, according to Merna, is a key factor in assessing risk. Whether a problem is perceived in terms of potential gains or losses will not be assessed as a simple mathematical calculation of the problem, but as a subjective fear, often linked to consequences of outcomes. Furthermore, he stated that risks are perceived by different stakeholders at different business level. Risk management process In construction projects each of the three primary targets of cost, time and quality is likely to be subject to risks. In terms of project implementation, managers need to be able to undertake or propose action which reduce or eliminate the effects of risks. To achieve this aim, a suggested systematic approach to the management of risk is followed: *Risk Identification: To identify the risk resource; *Risk Analysis: To quantify the effects; *Risk Response: To develop management response to risk Methodology In order to collect data and information for the research and examine the main questions raised, the following methodology will be possibly employed: Secondary Analysis Since direct observation is not very accessible in construction industry in China, secondary analysis is more preferable. Secondary analysis is the reanalysis of data that was originally compiled by another researcher for other purposes than the one the present researcher intends to use it for. The sources that I would use include archives, newspaper, and magazine articles. I will try to choose the sources which are more objective. I will adopt appropriate methods to find the useful data and information for my  research on risk management. Questionnaire Questionnaires are an inexpensive way to gather data from a potentially large number of respondents. Often they are the only feasible way to reach a number of reviewers large enough to allow statistically analysis of the results. For my study, the questionnaire is designed to collect data. The data will be compared with data collected from a similar survey conducted in 1979 by the American Society of Civil Engineers (ASCE). The purpose of this comparison is to identify ant trends in construction risk that will facilitate risk management. The reasons for me to choose questionnaire as research method as follows: Firstly, a Questionnaire can save resources and money. Although preparation may be costly, any data collection scheme will have similar preparation expenses. The administration cost per person of a questionnaire can be as low as postage and a few photocopies. Time is also an important resource that questionnaires can maximize. Secondly, Questionnaires are easy to administer confidentially. Thirdly, in studies that have resources to pursue other data collection strategies, questionnaires can be a useful confirmation tools. Thus, questionnaires are versatile, allowing the collection of both subjective and objective data through the use of open or closed format questions. Modern computers have only made the task of collecting and extracting valuable material more efficient. Individual Interviews Interviews will enable me to do most of the qualitative part of my research, and the information gained here is usually more realistic. I plan to interview some engineers, some project management team and some experienced project managers as well. In addition, Individual interviews by a way of structured questionnaire will also be used to collect information about the risk management perception. The interview is especially important for some proposed examining questions. I will also attempt to interview some  professional journal editors relating to construction industry. Case Study Case study research is the most common qualitative method used in risk management. As Yin (2002) defined, the scope of a case study as follows: A case study is an empirical inquiry that: *investigates a contemporary phenomenon within its real-life context, *especially when the boundaries between phenomenon and context are not clearly evident. For my research on risk management, I will choose some recent cases which relating to risk management as a way of identifying the perception and trends of risk management in construction industry in China. I will select both success and failing cases in Chinese construction industry. Proposed research Timetable I will spend about 4 months for my studies. I plan to spend the first month developing my proposal and methodology and completing the literature review. During this time, I will also try to obtain relevant published data and information. Then I plan to spend about one month in China obtaining statistics, doing questionnaires and interviews. I will spend the next two months in China so as to do some intensive fieldwork, and some follow-up jobs as well. May 2005 – June 2005 *Develop proposal and methodology and complete the literature review *Obtain relevant published statistics June 2005 – July 2005 *Intensive fieldwork in China *Analysis the statistics and data obtained July 2005 – August 2005 *Follow-up questionnaires and interviews and fieldwork in China August 2005 – September 2005 *Mainly work on the thesis (Details see attached Gantt chart) References: Al-Bahar, J. F., and Crandall, K. C. (1990) Systematic risk management approach for construction projects. Journal of Construction Engineering Management, 116~3, 533-546. China Statistical Yearbook, 2002, China Statistics Press. China Statistical Yearbook 2004, China Statistics Press. Lu, Y. J. (2003) Risk Management for Large-scale Infrastructure Projects in China, 11 Merna, A. and Smith, N. J. (1996) Projects procured by privately financial concession contracts, vol.1. Asia Law and Practice, Hong Kong. Raftery, J. (1994) Risk Analysis in Project Management. E & FN Spon, London. Smith, N. J. (2003) Appraisal, Risk and Uncertainty. 6, 42-43 Wehrung, D. A., Lee, K., Tse, D. K., and Vertinsky, I. B. (1988). Adjusting risky situations: A theoretical framework and empirical test. Journal of Risk Uncertainty, 2, 189-212. Yin, R. K. (2002) Case Study Research, Design and Methods, 3rd ed. Newbury Park, Sage Publications.